In two-step RT-PCR cDNA synthesis is carried out using random hexamers oligo-dT primers andor gene-specific primers which gives a mixture of cDNA molecules. Basics of real-time PCR 1 Real-time PCR primer design Good primer design is one of the most important parameters in real-time PCR.
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CDNAs thus synthesized are amplified using specific primers.
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. If you choose to design your own real. This is why many researchers choose to purchase TaqMan Assay productsprimers and probes for real-time PCR designed using a proven algorithm and trusted by scientists around the world. In two-step RT-PCR cDNA is synthesized in one reaction and an aliquot of the cDNA is then used for a.
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